Mikoto Uchiha (うちはミコト, Uchiha Mikoto) was a jōnin-level kunoichi of Konohagakure's Uchiha clan.
After marrying Fugaku Uchiha, she settled down to become a housewife, raising their sons Itachi and Sasuke. Just before her friend Kushina Uzumaki gave birth, she met with her and introduced Sasuke. She agreed with Kushina's hopes that their sons would become friends. After Kushina asked whether or not childbirth was painful, Mikoto laughed, having found the one thing that Kushina was seemingly afraid of.
During the Nine-Tails' Attack on Konoha, Itachi noted that she and Fugaku were noticeably absent before the beast struck. What she was doing is currently unknown.
During the massacre of the clan when Itachi confronted her and Fugaku, she was seen sitting beside her husband. When their son tried to explain himself, she simply told him that she understood before he killed them. This would later prompt Sasuke to want to avenge her death along with the rest of the clan.